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pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus
(Plasmid #61629)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 61629 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGreenII
  • Backbone size w/o insert (bp) 2580
  • Total vector size (bp) 12791
  • Vector type
    Plant Expression
  • Selectable markers
    Methothrexate

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin low (25 ug/ml)
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    mDII
  • Alt name
    Fragment of Arabidopsis IAA28, mutated to prevent auxin-dependent degradation
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    102
  • Promoter RPS5A

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    ntdTomato
  • Species
    Synthetic
  • Insert Size (bp)
    1518
  • GenBank ID
    KJ081243.1

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer M13_forward20_primer
  • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    DII
  • Alt name
    Fragment of Arabidopsis IAA28
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    102
  • Promoter RPS5A

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    n3xVenus
  • Alt name
    nuclear 3xVenus
  • Species
    Synthetic
  • Insert Size (bp)
    2255

Cloning Information for Gene/Insert 4

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer EGFP_N_primer
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus was a gift from Dolf Weijers (Addgene plasmid # 61629 ; http://n2t.net/addgene:61629 ; RRID:Addgene_61629)
  • For your References section:

    Reporters for sensitive and quantitative measurement of auxin response. Liao CY, Smet W, Brunoud G, Yoshida S, Vernoux T, Weijers D. Nat Methods. 2015 Mar;12(3):207-10. doi: 10.1038/nmeth.3279. Epub 2015 Feb 2. 10.1038/nmeth.3279 PubMed 25643149