Addgene - NM R2E2 GFP Plasmid Data

Plasmid 1208: NM R2E2 GFP

Gene/insert name:
sup35 NM domain
Alt name:
sup35
Insert size:
1990
Species:
S. cerevisiae (budding yeast)
GenBank ID:
AY553990
Entrez Gene:
SUP35 (YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)
Fusion protein or tag:
GFP
Terminal:
C terminal on insert
Mutation:
2 additional copies of second oligopeptide repeat in sup35 N domain
Vector backbone:
pRS316
(Search Vector Database)
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
4847
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
GFP cloned into CUP1-NM with SacI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Susan Lindquist
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Oligopeptide-repeat expansions modulate 'protein-only' inheritance in yeast. Liu et al (Nature 1999 Aug 5;400(6744):573-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1208" in your Materials and Methods section.