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pRAN473
(Plasmid #120813)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 120813 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRFP185
  • Backbone size w/o insert (bp) 6400
  • Total vector size (bp) 7950
  • Modifications to backbone
    Ptet::mCherryOpt-mcs
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Low copy in C. difficile following conjugation and selected with thiamphenicol
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
  • Alt name
    mCherryOpt-mcs
  • Species
    Synthetic
  • Insert Size (bp)
    1583
  • Promoter Ptet

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRAN473 was a gift from Craig Ellermeier & David S. Weiss (Addgene plasmid # 120813 ; http://n2t.net/addgene:120813 ; RRID:Addgene_120813)
  • For your References section:

    Use of mCherry Red fluorescent protein for studies of protein localization and gene expression in Clostridium difficile. Ransom EM, Ellermeier CD, Weiss DS. Appl Environ Microbiol. 2015 Mar;81(5):1652-60. doi: 10.1128/AEM.03446-14. Epub 2014 Dec 19. 10.1128/AEM.03446-14 PubMed 25527559