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DH10beta F' DOT sbcC (donor strain)
(Bacterial strain #12082)

No maps are available for this item.

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Bacterial Strain 12082 Bacteria in agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    F' Tra operon

Cloning Information

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

F' contains a transfer operon (tra) and oriT is replaced with the tetracycline-resistant gene (tetR). This modified F' can mobilize the donor plasmid but not itself. The bacterial strain is DH10beta F' DOT sbcC (donor host strain): mcrA delta(mrr-hsdRMS-mcrBC) phi80lacZdeltaM15 delta-lacX74 deoR recA1 endA1 ara-delta139 delta(ara, leu)7697 galU galK lambda- rpsL nupG tonA umuC::pir116-frt F'(lac+ pro+ delta-oriT::Tc) sbcC::Frt.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DH10beta F' DOT sbcC (donor strain) was a gift from Stephen Elledge (Addgene plasmid # 12082)
  • For your References section:

    A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells. Stegmeier F, Hu G, Rickles RJ, Hannon GJ, Elledge SJ. Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13212-7. 10.1073/pnas.0506306102 PubMed 16141338