Addgene - DH10beta F' DOT sbcC (donor strain) Plasmid Data

Bacterial strain 12082: DH10beta F' DOT sbcC (donor strain)

Gene/insert name:
F' Tra operon
Vector backbone:
na
(Search Vector Database)
Vector type:
Bacterial Expression
5' sequencing primer:
na List of Sequencing Primers
Bacterial resistance(s)
Tetracycline
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Supplemental document:
Elledge-pPRIME (application/pdf)
Principal Investigator:
Stephen Elledge
Terms and Licenses:
MTA

Comments:

F' contains a transfer operon (tra) and oriT is replaced with the tetracycline-resistant gene (tetR). This modified F' can mobilize the donor plasmid but not itself. The bacterial strain is DH10beta F' DOT sbcC (donor host strain): mcrA delta(mrr-hsdRMS-mcrBC) phi80lacZdeltaM15 delta-lacX74 deoR recA1 endA1 ara-delta139 delta(ara, leu)7697 galU galK lambda- rpsL nupG tonA umuC::pir116-frt F'(lac+ pro+ delta-oriT::Tc) sbcC::Frt.

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

Article: A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells. Stegmeier et al (Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13212-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12082" in your Materials and Methods section.