A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells.
Stegmeier F, Hu G, Rickles RJ, Hannon GJ, Elledge SJ
Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13212-7. PubMed Article
Stegmeier F, Hu G, Rickles RJ, Hannon GJ, Elledge SJ
Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13212-7. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 11655 | pPRIME-TET-GFP-recipient | 3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP expression |
| 11656 | pPRIME-TET-GIN-recipient | 3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP-IRES-Neo expression |
| 11657 | pPRIME-CMV-GFP-recipient | 3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling GFP expression |
| 11658 | pPRIME-CMV-dsRed-recipient | 3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling dsRed expression |
| 11659 | pPRIME-CMV-Neo-recipient | 3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling Neomycin expression |
| 11660 | pPRIME-CMV-LNGFR-recipient | 3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling low-affinity nerve growth factor receptor (LNGFR) expression |
| 11661 | pPRIME-TREX-GFP-recipient | 3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with Tet-responsive promoter (TREX) controlling GFP expression |
| 11662 | pPRIME-TET-GFP-FF3 | 3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a tetracycline-responsive promoter (TET) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase |
| 11663 | pPRIME-CMV-GFP-FF3 | 3rd generation lentiviral transfer vector. pPRIME cloning plasmid with CMV promoter controlling expression of GFP and miR30-based shRNA targeting firefly luciferase |
| 11664 | pPRIME-CMV-dsRed-FF3 | 3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of dsRed and miR30-based shRNA targeting firefly luciferase |
| 11665 | pPRIME-CMV-Neo-FF3 | 3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of Neomycin and miR30-based shRNA targeting firefly luciferase |
| 11666 | pPRIME-CMV-LNGFR-FF3 | 3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of LNGFR and miR30-based shRNA targeting firefly luciferase |
| 11667 | pPRIME-TREX-GFP-FF3 | 3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a Tet-responsive promoter (TREX) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase |
| 11675 | BUN21/pML300 (recipient strain) | |
| 12082 | DH10beta F' DOT sbcC (donor strain) |