Addgene - pPRIME-TET-GFP-FF3 Plasmid Data

Plasmid 11662: pPRIME-TET-GFP-FF3

Gene/insert name:
FF3
Alt name:
firefly luciferase hairpin
Insert size:
120
Species:
Firefly
Fusion protein or tag:
GFP
Terminal:
N terminal on backbone
Vector backbone:
pPRIME-TET-GFP
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
8226
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
EGFP-C List of Sequencing Primers
Bacterial resistance(s)
Chloramphenicol
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Stephen Elledge
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Comments:

TET promoter (minimal CMV promoter containing seven upstream Tet-operator sites) transcribes GFP and miR30-based shRNA targeting FF3. The FF3 hairpin can be replaced with any other hairpin by cloning into the EcoR1/Xho1 site.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells. Stegmeier et al (Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13212-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11662" in your Materials and Methods section.