Purpose(Empty Backbone) 3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP-IRES-Neo expression
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11656||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 9747
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberLow Copy
/ Fusion Proteins
- GFP (N terminal on backbone)
- IRES (N terminal on backbone)
- Neo (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
This recipient plasmid contains pheS Gly294 between the I-SceI sites, which can be selected against using Cl-Phe.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pPRIME-TET-GIN-recipient was a gift from Stephen Elledge (Addgene plasmid # 11656 ; http://n2t.net/addgene:11656 ; RRID:Addgene_11656)
For your References section:A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells. Stegmeier F, Hu G, Rickles RJ, Hannon GJ, Elledge SJ. Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13212-7. 10.1073/pnas.0506306102 PubMed 16141338