Purpose(Empty Backbone) 3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling low-affinity nerve growth factor receptor (LNGFR) expression
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11660||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 8744
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberLow Copy
/ Fusion Protein
- LNGFR (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
This recipient plasmid contains pheS Gly294 between the I-SceI sites, which can be selected against using Cl-Phe.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pPRIME-CMV-LNGFR-recipient was a gift from Stephen Elledge (Addgene plasmid # 11660 ; http://n2t.net/addgene:11660 ; RRID:Addgene_11660)
For your References section:A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells. Stegmeier F, Hu G, Rickles RJ, Hannon GJ, Elledge SJ. Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13212-7. 10.1073/pnas.0506306102 PubMed 16141338