Addgene - pCMV HDM2(NES-) Plasmid Data

Plasmid 12087: pCMV HDM2(NES-)

Gene/insert name:
HDM2
Species:
H. sapiens (human)
Entrez Gene:
MDM2 (ACTFS, HDMX, hdm2)
Mutation:
Nuclear-export sequence mutant (NES-): L205A, I208A
Vector backbone:
pCMV5
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4600
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
J. Xiao (Boston University)
Sequence: View sequences (1)
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: An intact HDM2 RING-finger domain is required for nuclear exclusion of p53. Boyd et al (Nat Cell Biol. 2000 Sep . 2(9):563-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12087" in your Materials and Methods section.