GFP-p53
(Plasmid #12091)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4730
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    p53
  • Species
    H. sapiens (human)
  • GenBank ID
    AAD28628.1
  • Entrez Gene
    TP53 (a.k.a. BCC7, LFS1, P53, TRP53)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The full cDNA sequence of the wild-type human p53 gene was amplified by PCR using a set of primers that introduced terminal PstI and BamHI sites. The amplified product was cloned into pEGFP/N1.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-p53 was a gift from Tyler Jacks (Addgene plasmid # 12091)
  • For your References section:

    An intact HDM2 RING-finger domain is required for nuclear exclusion of p53. Boyd SD, Tsai KY, Jacks T. Nat Cell Biol. 2000 Sep . 2(9):563-8. 10.1038/35023500 PubMed 10980695