Addgene - GFP-p53 Plasmid Data

Plasmid 12091: GFP-p53

Gene/insert name:
p53
Species:
H. sapiens (human)
GenBank ID:
AAD28628.1
Entrez Gene:
TP53 (BCC7, LFS1, P53, TRP53)
Fusion protein or tag:
GFP
Terminal:
C terminal on backbone
Vector backbone:
pEGFP-N1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4730
Cloning site 5':
PstI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
EGFP-N
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (6)
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Comments:

The full cDNA sequence of the wild-type human p53 gene was amplified by PCR using a set of primers that introduced terminal PstI and BamHI sites. The amplified product was cloned into pEGFP/N1.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: An intact HDM2 RING-finger domain is required for nuclear exclusion of p53. Boyd et al (Nat Cell Biol. 2000 Sep . 2(9):563-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12091" in your Materials and Methods section.