Addgene - pcDNA3 flag p53 Plasmid Data

Plasmid 10838: pcDNA3 flag p53

Gene/insert name:
p53
Insert size:
1200
Species:
H. sapiens (human)
Entrez Gene:
TP53 (BCC7, LFS1, P53, TRP53)
Fusion protein or tag:
flag
Terminal:
N terminal on backbone
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5500
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
BGH-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (3)
Principal Investigator:
Thomas Roberts
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Induction of p53-independent apoptosis by simian virus 40 small t antigen. Gjoerup et al (J Virol. 2001 Oct . 75(19):9142-55. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10838" in your Materials and Methods section.