MG15-luc (mut p53 binding sites)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16443||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Gene/Insert namep53-binding site (mutant)
Entrez GeneTP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (destroyed during cloning)
- 3′ cloning site EcoRV (destroyed during cloning)
- 5′ sequencing primer M13pUC-Fwd
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
MG15-Luc was cloned by inserting a HindIII-EcoRI fragment containing p53-binding elements (Kern et al Science 1992 May 8;256(5058):827-30) into the Hindlll-EcoRI sites of pBluescript II SK(+). A 200 bp EcoRI-BamHI fragment containing the polyoma promoter, and a 2.6 kb SacI luciferase cassette without promoter elements, were then cloned downstream.
MG15 contains 15 copies of a mutated p53-binding sequence. Addgene has confirmed the presence of 8 of these copies.
Direct repeats of the oligonucleotide MG (5'-CCTTAATGGACTTTAATGG-
3') were used for the
p53 nonbinding control sequences.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MG15-luc (mut p53 binding sites) was a gift from Bert Vogelstein (Addgene plasmid # 16443 ; http://n2t.net/addgene:16443 ; RRID:Addgene_16443)
For your References section:WAF1, a potential mediator of p53 tumor suppression. el-Deiry WS, Tokino T, Velculescu VE, Levy DB, Parsons R, Trent JM, Lin D, Mercer WE, Kinzler KW, Vogelstein B. Cell. 1993 Nov 19. 75(4):817-25. 10.1016/0092-8674(93)90500-P PubMed 8242752