PG13-luc (wt p53 binding sites)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16442||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript II SK + luc
- Backbone size w/o insert (bp) 5800
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Gene/Insert namep53-binding site
Entrez GeneTP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
PG13-Luc was cloned by inserting a HindIII-EcoRI fragment containing p53-binding elements (Kern et al Science 1992 May 8;256(5058):827-30) into the Hindlll-EcoRI sites of pBluescript II SK(+). A 200 bp EcoRI-BamHI fragment containing the polyoma promoter, and a 2.6 kb SacI luciferase cassette without promoter elements, were then cloned downstream.
PG13 contains 13 copies of the p53-binding consensus sequence.
Direct repeats of the oligonucleotide PG (5'-CCAGGCAAGTCCAGGCAGG-3') were used for the
p53 binding sequences.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PG13-luc (wt p53 binding sites) was a gift from Bert Vogelstein (Addgene plasmid # 16442 ; http://n2t.net/addgene:16442 ; RRID:Addgene_16442)
For your References section:WAF1, a potential mediator of p53 tumor suppression. el-Deiry WS, Tokino T, Velculescu VE, Levy DB, Parsons R, Trent JM, Lin D, Mercer WE, Kinzler KW, Vogelstein B. Cell. 1993 Nov 19. 75(4):817-25. 10.1016/0092-8674(93)90500-P PubMed 8242752