pCMV-Neo-Bam p53 wt
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16434||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerVogelstein Lab
- Backbone size w/o insert (bp) 6600
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
SpeciesH. sapiens (human)
Insert Size (bp)1800
Entrez GeneTP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
A 1.8kb XbaI fragment encoding wild-type p53 (from nucleotide -130 to 1671 relative to the ATG) was blunt-ended and ligated to BamHI linkers. This was then cloned into the unique BamHI site in the pCMV-Neo-Bam vector. This plasmid is also known as pC53-SN3.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-Neo-Bam p53 wt was a gift from Bert Vogelstein (Addgene plasmid # 16434 ; http://n2t.net/addgene:16434 ; RRID:Addgene_16434)
For your References section:Suppression of human colorectal carcinoma cell growth by wild-type p53. Baker SJ, Markowitz S, Fearon ER, Willson JK, Vogelstein B. Science. 1990 Aug 24. 249(4971):912-5. 10.1126/science.2144057 PubMed 2144057