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Plasmid 16440: pCMV-Neo-Bam
  • Contains two independent transcription units--a CMV promoter/enhancer upstream of a MCS and a HSV thymidine kinase promoter/enhancer upstream of the neomycin resistance gene.

  • None

  • pCMV-Neo-Bam
    (Search Vector Database)

  • Vogelstein Lab

  • Mammalian Expression

  • 6600

  • BamHI

  • No

  • BamHI

  • No

  • Bglob-intron-F List of Sequencing Primers

  • Bglob-pA-R

  • Ampicillin

  • DH5alpha

  • 37

  • Unknown

  • Neomycin

  • View sequences (2)
  • View map

  • Bert Vogelstein

  • UBMTA

Comments: 

The vector pCMV-Neo-Bam was engineered to contain two independent transcription units. The first unit is comprised of a CMV promoter/enhancer upstream of a multiple cloning site, and splice and polyA sites to ensure appropriate processing. The second transcription unit includes a herpes simplex virus (HSV) thymidine kinase promoter/enhancer upstream of the neomycin resistance gene.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Suppression of human colorectal carcinoma cell growth by wild-type p53. Baker et al (Science. 1990 Aug 24. 249(4971):912-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16440" in your Materials and Methods section.