Addgene - pCMV-Neo-Bam Plasmid Data

Plasmid 16440: pCMV-Neo-Bam

Gene/insert name:
None
Vector backbone:
pCMV-Neo-Bam
(Search Vector Database)
Backbone manufacturer:
Vogelstein Lab
Vector type:
Mammalian Expression
Backbone size (bp):
6600
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
Bglob-intron-F List of Sequencing Primers
3' sequencing primer:
Bglob-pA-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Neomycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Bert Vogelstein
Terms and Licenses:
MTA

Comments:

The vector pCMV-Neo-Bam was engineered to contain two independent transcription units. The first unit is comprised of a CMV promoter/enhancer upstream of a multiple cloning site, and splice and polyA sites to ensure appropriate processing. The second transcription unit includes a herpes simplex virus (HSV) thymidine kinase promoter/enhancer upstream of the neomycin resistance gene.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Suppression of human colorectal carcinoma cell growth by wild-type p53. Baker et al (Science. 1990 Aug 24. 249(4971):912-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16440" in your Materials and Methods section.