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pCMV-Neo-Bam
(Plasmid #16440)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCMV-Neo-Bam
  • Backbone manufacturer
    Vogelstein Lab
  • Backbone size (bp) 6600
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer Bglob-intron-F
  • 3′ sequencing primer Bglob-pA-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

The vector pCMV-Neo-Bam was engineered to contain two independent transcription units. The first unit is comprised of a CMV promoter/enhancer upstream of a multiple cloning site, and splice and polyA sites to ensure appropriate processing. The second transcription unit includes a herpes simplex virus (HSV) thymidine kinase promoter/enhancer upstream of the neomycin resistance gene.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Neo-Bam was a gift from Bert Vogelstein (Addgene plasmid # 16440)
  • For your References section:

    Suppression of human colorectal carcinoma cell growth by wild-type p53. Baker SJ, Markowitz S, Fearon ER, Willson JK, Vogelstein B. Science. 1990 Aug 24. 249(4971):912-5. 10.1126/science.2144057 PubMed 2144057