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pCB6-HFE-EGFP
(Plasmid #12104)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12104 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCB6
  • Backbone manufacturer
    Ira Mellman, Yale University
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HFE
  • Alt name
    hemochromatosis protein
  • Alt name
    MHC class I-like protein HFE
  • Alt name
    hereditary hemochromatosis protein HLA-H
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1044
  • GenBank ID
    Q30201
  • Entrez Gene
    HFE (a.k.a. HFE1, HH, HLA-H, MVCD7, TFQTL2)
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCB6-HFE-EGFP was a gift from Pamela Bjorkman (Addgene plasmid # 12104 ; http://n2t.net/addgene:12104 ; RRID:Addgene_12104)
  • For your References section:

    HFE and transferrin directly compete for transferrin receptor in solution and at the cell surface. Giannetti AM, Bjorkman PJ. J Biol Chem. 2004 Jun 11. 279(24):25866-75. 10.1074/jbc.M401467200 PubMed 15056661