Addgene - pMB84 (Nf2 C-term) Plasmid Data

Plasmid 12169: pMB84 (Nf2 C-term)

Gene/insert name:
Nf2 C-terminal half
Alt name:
Nf2
Insert size:
836
Species:
M. musculus (mouse)
Entrez Gene:
Nf2 (RP23-280J3.6, merlin)
Mutation:
C-terminal half of Nf2 cDNA
Vector backbone:
pCR-Blunt II-TOPO
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression, Cre/Lox
Backbone size w/o insert (bp):
3500
Cloning method:
TOPO Cloning
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Comments:

In situ probe for Nf2.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The Nf2 tumor suppressor regulates cell-cell adhesion during tissue fusion. McLaughlin et al (Proc Natl Acad Sci U S A. 2007 Feb 27. 104(9):3261-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12169" in your Materials and Methods section.