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pMB84 (Nf2 C-term)
(Plasmid #12169)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCR-Blunt II-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3500
  • Vector type
    Mammalian Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Nf2 C-terminal half
  • Alt name
    Nf2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    836
  • Mutation
    C-terminal half of Nf2 cDNA
  • Entrez Gene
    Nf2 (a.k.a. RP23-280J3.6, merlin)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

In situ probe for Nf2.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMB84 (Nf2 C-term) was a gift from Tyler Jacks (Addgene plasmid # 12169)
  • For your References section:

    The Nf2 tumor suppressor regulates cell-cell adhesion during tissue fusion. McLaughlin ME, Kruger GM, Slocum KL, Crowley D, Michaud NA, Huang J, Magendantz M, Jacks T. Proc Natl Acad Sci U S A. 2007 Feb 27. 104(9):3261-6. 10.1073/pnas.0700044104 PubMed 17360635