Addgene - pL3-TRE-LucGFP-2L Plasmid Data

Gene/insert name:
Luciferase-EGFP
Vector backbone:
pL3-TRE-MCS-polyA-2L
(Search Vector Database)
Backbone manufacturer:
Wahl lab
Vector type:
Mammalian Expression, Cre/Lox
Backbone size w/o insert:
7117
Cloning site 5':
BamHI/BglII
Site destroyed during cloning:
Yes
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
See map List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
LucGFP fragment was cloned from pLuciferase-EGFP vector (gift of D. Buscher).
Sequence: View sequences (2)
Principal Investigator:
Geoff Wahl
Terms and Licenses:
MTA
Luciferase Limited Use Label License
Tet Systems Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Reproducible doxycycline-inducible transgene expression at specific loci generated by Cre-recombinase mediated cassette exchange. Wong et al (Nucleic Acids Res. 2005 . 33(17):e147. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11685" in your Materials and Methods section.