Addgene - RV-L3-HyTK-2L Plasmid Data

Plasmid 11684: RV-L3-HyTK-2L

Gene/insert name:
None
Vector backbone:
pCFB-EGSH
(Search Vector Database)
Backbone manufacturer:
Stratagene
Vector type:
Mammalian Expression, Retroviral, Cre/Lox
Backbone size (bp):
9177
Modifications to Backbone:
new loxP site L3 (see paper)
5' sequencing primer:
See map List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Hygromycin
Person or lab that originally
cloned the gene/insert:
H1HyTK2L cassette was from S. Fiering. It was modified to become L3HyTK2L.
Sequence: View sequences (2)
Principal Investigator:
Geoff Wahl
Terms and Licenses:
MTA

Comments:

L3HyTK2L was constructed by replacing L1 in L1HyTK2L with L3. This cassette was cloned into the backbone by inserting L3HyTK2L restrcted with NotI and XbaI into pCFB-EGSH digested with the same enzymes.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Reproducible doxycycline-inducible transgene expression at specific loci generated by Cre-recombinase mediated cassette exchange. Wong et al (Nucleic Acids Res. 2005 . 33(17):e147. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11684" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

11719: pL3-TRE-MCS-polyA-2L

11685: pL3-TRE-LucGFP-2L

17736: pOG231

13772: pCAFNF-GFP

13770: pCALNL-GFP