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RV-L3-HyTK-2L
(Plasmid #11684)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11684 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCFB-EGSH
  • Backbone manufacturer
    Stratagene
  • Backbone size (bp) 9177
  • Modifications to backbone
    new loxP site L3 (see paper)
  • Vector type
    Mammalian Expression, Retroviral, Cre/Lox
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    H1HyTK2L cassette was from S. Fiering. It was modified to become L3HyTK2L.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

L3HyTK2L was constructed by replacing L1 in L1HyTK2L with L3. This cassette was cloned into the backbone by inserting L3HyTK2L restrcted with NotI and XbaI into pCFB-EGSH digested with the same enzymes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RV-L3-HyTK-2L was a gift from Geoff Wahl (Addgene plasmid # 11684 ; http://n2t.net/addgene:11684 ; RRID:Addgene_11684)
  • For your References section:

    Reproducible doxycycline-inducible transgene expression at specific loci generated by Cre-recombinase mediated cassette exchange. Wong ET, Kolman JL, Li YC, Mesner LD, Hillen W, Berens C, Wahl GM. Nucleic Acids Res. 2005 . 33(17):e147. 10.1093/nar/gni145 PubMed 16204450