|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11684||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 9177
Modifications to backbonenew loxP site L3 (see paper)
Vector typeMammalian Expression, Retroviral, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer See map (Common Sequencing Primers)
L3HyTK2L was constructed by replacing L1 in L1HyTK2L with L3. This cassette was cloned into the backbone by inserting L3HyTK2L restrcted with NotI and XbaI into pCFB-EGSH digested with the same enzymes.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:RV-L3-HyTK-2L was a gift from Geoff Wahl (Addgene plasmid # 11684 ; http://n2t.net/addgene:11684 ; RRID:Addgene_11684)
For your References section:Reproducible doxycycline-inducible transgene expression at specific loci generated by Cre-recombinase mediated cassette exchange. Wong ET, Kolman JL, Li YC, Mesner LD, Hillen W, Berens C, Wahl GM. Nucleic Acids Res. 2005 . 33(17):e147. 10.1093/nar/gni145 PubMed 16204450