Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17736||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepBluescript II KS-
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameNLS-Cre recombinase
Insert Size (bp)1800
- Cloning method Restriction Enzyme
- 5′ cloning site SacII (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer M13-R (Common Sequencing Primers)
The Cre expression plasmid pOG231 was prepared by fusing a Cre coding sequence modified from pIC-Cre, and containing the same translation start and nuclear localization signal, to the synthetic intron and cytomegalovirus promoter of pOG44
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pOG231 was a gift from Geoff Wahl (Addgene plasmid # 17736 ; http://n2t.net/addgene:17736 ; RRID:Addgene_17736)
For your References section:Protamine-Cre recombinase transgenes efficiently recombine target sequences in the male germ line of mice, but not in embryonic stem cells. O'Gorman S, Dagenais NA, Qian M, Marchuk Y. Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14602-7. PubMed 9405659