KJ901: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS
Purpose(Empty Backbone) pMAGIC R4-R3 entry plasmid, contains 2x NLS scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||121835||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepDONR221 P4r-P3r
- Backbone size (bp) 2664
Vector typeSynthetic Biology ; pMAGIC Gateway Entry Plasmid
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name2x NLS (open)
Insert Size (bp)141
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
SrfI/PmeI fragment can be replaced with dCas9 variant. NcoI/BamHI fragment (2xNLS) can be replaced with a gene of interest for use with pMVP components.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:KJ901: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS was a gift from Christopher Newgard (Addgene plasmid # 121835 ; http://n2t.net/addgene:121835 ; RRID:Addgene_121835)
For your References section:Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing. Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, Newgard CB. Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286. 10.1093/nar/gky1286 PubMed 30590691