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pCF709
(Plasmid #121891)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 121891 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEF1a
  • Backbone size (bp) 10284
  • Vector type
    Lentiviral
  • Promoter EF1a core promoter
  • Selectable markers
    bleoR gene: antibiotic binding protein

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    another resistance
  • Copy number
    High Copy

Cloning Information

  • Cloning method Unknown

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCF709 was a gift from David Savage (Addgene plasmid # 121891 ; http://n2t.net/addgene:121891 ; RRID:Addgene_121891)
  • For your References section:

    Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch. Oakes BL, Nadler DC, Flamholz A, Fellmann C, Staahl BT, Doudna JA, Savage DF. Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528. 10.1038/nbt.3528 PubMed 27136077