Addgene - pJ3M-Mst2 Plasmid Data

Plasmid 12205: pJ3M-Mst2

Gene/insert name:
Mst2
Insert size:
2000
Species:
H. sapiens (human)
Entrez Gene:
STK3 (KRS1, MST2)
Fusion protein or tag:
Myc
Terminal:
N terminal on backbone
Vector backbone:
pJ3M
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
3500
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
KpnI
Site destroyed during cloning:
No
5' sequencing primer:
SV40pro-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Jonathan Chernoff
Terms and Licenses:
MTA

Comments:

Addgene's quality control sequence shows a mutation at amino acid E331D of STK3. This mutation is outside the kinase domain and the SARAH dimerization domain, and hence should not affect plasmid function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The Ste20-like protein kinase, Mst1, dimerizes and contains an inhibitory domain. Creasy et al (J Biol Chem. 1996 Aug 30. 271(35):21049-53. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12205" in your Materials and Methods section.