Addgene - p16-1 hTRF2 Plasmid Data

Plasmid 12299: p16-1 hTRF2

Gene/insert name:
hTRF2
Alt name:
human TTAGGG repeat binding factor 2
Alt name:
1641
Insert size:
2900
Species:
H. sapiens (human)
Entrez Gene:
TERF2 (TRBF2, TRF2)
Mutation:
A434 deleted; There is a stop codon in the 5'UTR
Vector backbone:
N/A
(Search Vector Database)
Backbone manufacturer:
Stratagene
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
2900
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
T3 List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Titia de Lange
Terms and Licenses:
MTA

Comments:

Please note that this plasmid contains a 3 nucleotide deletion in the hTRF2 sequence when compared to GenBank ID NM_005652.3 which results in the deletion of amino acid A434. The deletion was present in the original construct described in the associated publication below and is not known to affect TRF2 activity.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Human telomeres contain two distinct Myb-related proteins, TRF1 and TRF2. Broccoli et al (Nat Genet. 1997 Oct . 17(2):231-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12299" in your Materials and Methods section.