eGFP STAT1 WT
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12301||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)2300
Entrez GeneSTAT1 (a.k.a. CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91)
/ Fusion Protein
- eGFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer EGFP-C (5'-CATGGTCCTGCTGGAGTTCGTG-3') (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
The STAT1 full-size coding region was amplified using total cDNA from SK-NEP-1 cells and cloned into pEGFP-C1. The hSTAT1 insert contains an N372D mutation compared to all available reference sequences.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:eGFP STAT1 WT was a gift from Alan Perantoni (Addgene plasmid # 12301 ; http://n2t.net/addgene:12301 ; RRID:Addgene_12301)
For your References section:Serine-phosphorylated STAT1 is a prosurvival factor in Wilms' tumor pathogenesis. Timofeeva OA, Plisov S, Evseev AA, Peng S, Jose-Kampfner M, Lovvorn HN, Dome JS, Perantoni AO. Oncogene. 2006 Jun 26. ():. 10.1038/sj.onc.1209742 PubMed 16799645