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eGFP STAT1 WT
(Plasmid #12301)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12301 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    STAT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2300
  • GenBank ID
    NM_007315
  • Entrez Gene
    STAT1 (a.k.a. CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91)
  • Tag / Fusion Protein
    • eGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer EGFP-C (5'-CATGGTCCTGCTGGAGTTCGTG-3')
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The STAT1 full-size coding region was amplified using total cDNA from SK-NEP-1 cells and cloned into pEGFP-C1. The hSTAT1 insert contains an N372D mutation compared to all available reference sequences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    eGFP STAT1 WT was a gift from Alan Perantoni (Addgene plasmid # 12301)
  • For your References section:

    Serine-phosphorylated STAT1 is a prosurvival factor in Wilms' tumor pathogenesis. Timofeeva OA, Plisov S, Evseev AA, Peng S, Jose-Kampfner M, Lovvorn HN, Dome JS, Perantoni AO. Oncogene. 2006 Jun 26. ():. 10.1038/sj.onc.1209742 PubMed 16799645