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eGFP STAT1 Y701F
(Plasmid #12302)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12302 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    STAT1 Y701F
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2300
  • Mutation
    Y701F
  • GenBank ID
    NM_007315
  • Entrez Gene
    STAT1 (a.k.a. CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91)
  • Tag / Fusion Protein
    • eGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EGFP-C (5'-CATGGTCCTGCTGGAGTTCGTG-3')
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The STAT1 full-size coding region was amplified using total cDNA from SK-NEP-1 cells and cloned into pEGFP-C1. Y701F mutation made using QuikChange mutagenesis kit. The STAT1 insert contains a D372N mutation compared to all available reference sequences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    eGFP STAT1 Y701F was a gift from Alan Perantoni (Addgene plasmid # 12302 ; http://n2t.net/addgene:12302 ; RRID:Addgene_12302)
  • For your References section:

    Serine-phosphorylated STAT1 is a prosurvival factor in Wilms' tumor pathogenesis. Timofeeva OA, Plisov S, Evseev AA, Peng S, Jose-Kampfner M, Lovvorn HN, Dome JS, Perantoni AO. Oncogene. 2006 Jun 26. ():. 10.1038/sj.onc.1209742 PubMed 16799645