eGFP STAT1 Y701F
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12302||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameSTAT1 Y701F
SpeciesH. sapiens (human)
Insert Size (bp)2300
Entrez GeneSTAT1 (a.k.a. CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91)
/ Fusion Protein
- eGFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer EGFP-C (5'-CATGGTCCTGCTGGAGTTCGTG-3') (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Article Citing this Plasmid
The STAT1 full-size coding region was amplified using total cDNA from SK-NEP-1 cells and cloned into pEGFP-C1. Y701F mutation made using QuikChange mutagenesis kit. The STAT1 insert contains a D372N mutation compared to all available reference sequences.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:eGFP STAT1 Y701F was a gift from Alan Perantoni (Addgene plasmid # 12302 ; http://n2t.net/addgene:12302 ; RRID:Addgene_12302)
For your References section:Serine-phosphorylated STAT1 is a prosurvival factor in Wilms' tumor pathogenesis. Timofeeva OA, Plisov S, Evseev AA, Peng S, Jose-Kampfner M, Lovvorn HN, Dome JS, Perantoni AO. Oncogene. 2006 Jun 26. ():. 10.1038/sj.onc.1209742 PubMed 16799645
Map uploaded by the depositor.