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pAG/MNase
(Plasmid #123461)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 123461 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    JM101
  • Growth instructions
    Amplify plasmid in a standard cloning strain of bacteria, expression for purification should be in JM101.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Protein A-Protein G-Micrococcal Nuclease
  • Alt name
    pAG-MNase
  • Alt name
    pAG/MN
  • Alt name
    pAG(ERH)_MNase
  • Insert Size (bp)
    1218
  • Promoter Lac
  • Tags / Fusion Proteins
    • 6xHIS (C terminal on insert)
    • HA (C terminal on insert)

Cloning Information

Resource Information

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/569129v1 for BioRxiv preprint

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG/MNase was a gift from Steven Henikoff (Addgene plasmid # 123461)
  • For your References section:

    Improved CUT&RUN chromatin profiling tools. Meers MP, Bryson TD, Henikoff JG, Henikoff S. Elife. 2019 Jun 24;8. pii: 46314. doi: 10.7554/eLife.46314. 10.7554/eLife.46314 PubMed 31232687