|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||86973||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2661
- Total vector size (bp) 3534
Vector typeBacterial Expression
Growth in Bacteria
Gene/Insert nameprotein_A fused to Staphylococcus aureus microccocal nuclease (MNase)
Insert Size (bp)927
GenBank IDGenbank P00644 GenBank: CP018205.1
This plasmid expresses a pA-MN fusion protein in E. coli. The MN coding sequence was cloned into pK19SEVII (Giraud-Panis et al., 1995). The pA segment contains 2 IgG binding domains of staphylococcal protein A and was fused to MN with a linker sequence of DDDKEF.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pK19pA-MN was a gift from Ulrich Laemmli (Addgene plasmid # 86973 ; http://n2t.net/addgene:86973 ; RRID:Addgene_86973)
For your References section:ChIC and ChEC; genomic mapping of chromatin proteins. Schmid M, Durussel T, Laemmli UK. Mol Cell. 2004 Oct 8;16(1):147-57. 10.1016/j.molcel.2004.09.007 PubMed 15469830