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(Plasmid #86973)


Item Catalog # Description Quantity Price (USD)
Plasmid 86973 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 2661
  • Total vector size (bp) 3534
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    protein_A fused to Staphylococcus aureus microccocal nuclease (MNase)
  • Species
    Staphylococcus aureus
  • Insert Size (bp)
  • GenBank ID
    Genbank P00644 GenBank: CP018205.1

Resource Information

Depositor Comments

This plasmid expresses a pA-MN fusion protein in E. coli. The MN coding sequence was cloned into pK19SEVII (Giraud-Panis et al., 1995). The pA segment contains 2 IgG binding domains of staphylococcal protein A and was fused to MN with a linker sequence of DDDKEF.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pK19pA-MN was a gift from Ulrich Laemmli (Addgene plasmid # 86973 ; ; RRID:Addgene_86973)
  • For your References section:

    ChIC and ChEC; genomic mapping of chromatin proteins. Schmid M, Durussel T, Laemmli UK. Mol Cell. 2004 Oct 8;16(1):147-57. 10.1016/j.molcel.2004.09.007 PubMed 15469830