Addgene - RCAS IkB K->A Plasmid Data

Gene/insert name:
IkB alpha K->A
Alt name:
I kappa B alpha K->A mutant
Alt name:
I kappa B alpha
Species:
M. musculus (mouse)
Entrez Gene:
Nfkbia (AI462015, Nfkbi)
Mutation:
Lysines 21, 22, and 67 are mutated to alanine. (The lysines are essential for targeting IkB for signal-induced degradation by the ubiquitin-proteasome system.)
Vector backbone:
RCAS
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert:
11500
5' sequencing primer:
RCAS-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Inder Verma
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12408" in your Materials and Methods section.