Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pEF1a_ TET1 CXXC only
(Plasmid #124084)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 124084 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEF1 alpha
  • Backbone size w/o insert (bp) 6112
  • Total vector size (bp) 10519
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TET1 - CXXC domain
  • Alt name
    Ten Eleven Translocation methylcytosine dioxygenase 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4407
  • Entrez Gene
    TET1 (a.k.a. CXXC6, LCX, bA119F7.1)
  • Promoter EF1 alpha
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Plasmid expressing wild type full length form of TET1 was taken from addgene (#49792)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF1a_ TET1 CXXC only was a gift from Silvia Monticelli (Addgene plasmid # 124084 ; http://n2t.net/addgene:124084 ; RRID:Addgene_124084)
  • For your References section:

    The contribution of active and passive mechanisms of 5mC and 5hmC removal in human T lymphocytes is differentiation- and activation-dependent. Vincenzetti L, Leoni C, Chirichella M, Kwee I, Monticelli S. Eur J Immunol. 2019 Jan 30. doi: 10.1002/eji.201847967. 10.1002/eji.201847967 PubMed 30698829