NFATc3-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||124288||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepSpCas9(BB)-2A-Puro (PX459) V2.0
Backbone manufacturerFeng Zhang (Addgene plasmid # 62988)
- Backbone size w/o insert (bp) 9200
Growth in Bacteria
Growth Strain(s)NEB Stable
Gene/Insert nameNFATc3 nuclear factor of activated T cells
SpeciesH. sapiens (human)
Insert Size (bp)21
Entrez GeneNFATC3 (a.k.a. NF-AT4c, NFAT4, NFATX)
- Cloning method Restriction Enzyme
- 5′ cloning site BbsI (destroyed during cloning)
- 3′ cloning site BbsI (destroyed during cloning)
- 5′ sequencing primer U6-fw: GGGCCTATTTCCCATGATTCCTTC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Plus "G" was routinely added because U6 promoter prefers "G" as the first transcribed nucleotide. gRNA sequence contains at least 3 mismatches, and was selected by choosing the highest scored sequence from different programs and databases.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:NFATc3-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0) was a gift from Katalin Josvay & Csaba Vizler (Addgene plasmid # 124288 ; http://n2t.net/addgene:124288 ; RRID:Addgene_124288)