M51 Super 8x FOPFlash (TOPFlash mutant)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12457||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4839
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namemutant TCF/LEF binding sites
Alt namebeta catenin reporter
Insert Size (bp)134
Mutationmutant TCF/LEF binding sites
Entrez GeneCtnnb1 (a.k.a. Bfc, Catnb, Mesc)
- Cloning method Restriction Enzyme
- 5′ cloning site Asp718 (not destroyed)
- 3′ cloning site XmaI (not destroyed)
- 5′ sequencing primer RVPrimer3
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
This is a CONTROL luciferase reporter with the TCF/LEF sites of Super8XTOPflash (construct M50, Addgene Plasmid #12456) being mutated.
There are 6 mutated TCF/LEF binding sites that were cloned into the pGL3 vector (Promega), which contains a minimal promoter followed by a firefly luciferase open reading frame. This 134bp fragment was gene synthesized and cloned into the ASP718 and XMA-1 sites of the vector.
Note: This plasmid was published as M51 Super 8x FOPFlash, but the plasmid actually contains 6 TCF/LEF sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:M51 Super 8x FOPFlash (TOPFlash mutant) was a gift from Randall Moon (Addgene plasmid # 12457 ; http://n2t.net/addgene:12457 ; RRID:Addgene_12457)
For your References section:Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements. Veeman MT, Slusarski DC, Kaykas A, Louie SH, Moon RT. Curr Biol. 2003 Apr 15. 13(8):680-5. 10.1016/S0960-9822(03)00240-9 PubMed 12699626