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pRL-SV40P
(Plasmid #27163)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27163 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRL-TK
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4045
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SV40 promoter
  • Tag / Fusion Protein
    • Renilla Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer SV40pro-F
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

pRL-SV40P was generated by placing the simian virus 40 (SV40) promoter from pGL3-Promoter (Promega) into pRL-TK such that the SV40 promoter is driving the Renilla luciferase gene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRL-SV40P was a gift from Ron Prywes (Addgene plasmid # 27163 ; http://n2t.net/addgene:27163 ; RRID:Addgene_27163)
  • For your References section:

    Serum-induced expression of the cdc25A gene by relief of E2F-mediated repression. Chen X, Prywes R. Mol Cell Biol. 1999 Jul . 19(7):4695-702. PubMed 10373518