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pGEX-mUbp43-His
(Plasmid #12458)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12458 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-4T3
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UBP43
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Usp18 (a.k.a. 1110058H21Rik, AW047653, UBP4, UBP43, Ubp15)
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer pGEX 5'
  • 3′ sequencing primer pGEX 3'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-mUbp43-His was a gift from Dong-Er Zhang (Addgene plasmid # 12458 ; http://n2t.net/addgene:12458 ; RRID:Addgene_12458)
  • For your References section:

    UBP43 (USP18) specifically removes ISG15 from conjugated proteins. Malakhov MP, Malakhova OA, Kim KI, Ritchie KJ, Zhang DE. J Biol Chem. 2002 Mar 22. 277(12):9976-81. 10.1074/jbc.M109078200 PubMed 11788588