Addgene - pCAGGS-HA-hUBE1L Plasmid Data

Plasmid 12438: pCAGGS-HA-hUBE1L

Gene/insert name:
UBE1L
Species:
H. sapiens (human)
Entrez Gene:
UBA7 (D8, MGC12713, UBA1B, UBE1L, UBE2)
Fusion protein or tag:
HA
Terminal:
N terminal on insert
Vector backbone:
pCAGGS
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4790
Cloning site 5':
EcoRI?
Site destroyed during cloning:
No
5' sequencing primer:
pCAG-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
UBE1L cDNA was provided by Charles Buys and subcloned into pCAGGS
Sequence: View sequences (1)
Principal Investigator:
Dong-Er Zhang
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Interferon-inducible ubiquitin E2, Ubc8, is a conjugating enzyme for protein ISGylation. Kim et al (Mol Cell Biol. 2004 Nov . 24(21):9592-600. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12438" in your Materials and Methods section.