Addgene - pcDNA-hISG15 Plasmid Data

Plasmid 12447: pcDNA-hISG15

Gene/insert name:
ISG15 fragment
Alt name:
ISG15
Species:
H. sapiens (human)
Entrez Gene:
ISG15 (G1P2, IFI15, IP17, UCRP, hUCRP)
Mutation:
ISG15 up to LRLRGG
Vector backbone:
pcDNA3.1
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5428
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Dong-Er Zhang
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Interferon-inducible ubiquitin E2, Ubc8, is a conjugating enzyme for protein ISGylation. Kim et al (Mol Cell Biol. 2004 Nov . 24(21):9592-600. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12447" in your Materials and Methods section.