PH-Btk-mRuby2
(Plasmid #124657)

• Purpose: PI(3,4,5)P3 probe. PH domain of BTK fused with mRuby2
• Depositing Lab: Cheng-han Yu
• Publication: Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635. doi: 10.1091/mbc.E18-06-0398. Epub 2019 Jan 2.

Backbone

• Vector backbone: pmRuby2-N1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BTK
• Alt name: PH domain of Bruton tyrosine kinase (aa 1–177)
• Species: H. sapiens (human)
• Mutation: contains only the PH domain of Bruton tyrosine kinase (aa 1–177)
• GenBank ID: NM_000061.2
• Entrez Gene: BTK (a.k.a. AGMX1, AT, ATK, BPK, IGHD3, IMD1, PSCTK1, XLA)
• Tag / Fusion Protein:
  • mRuby2 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry