PurposeExpresses the proton pump rhodopsin in mammalian cells under CAG promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||124792||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerMichael Lin lab (Addgene plasmid 52519)
- Backbone size w/o insert (bp) 6105
- Total vector size (bp) 7779
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1680
- Promoter CAG
- Cloning method Gibson Cloning
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Addgene NGS found a single nucleotide deletion in the Kozak sequence upstream of Char-BFP2 compared to a reference sequence provided by the depositing lab
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.1-Char-BFP2-TSERex was a gift from Yulong Li (Addgene plasmid # 124792 ; http://n2t.net/addgene:124792 ; RRID:Addgene_124792)
For your References section:PARIS, an optogenetic method for functionally mapping gap junctions. Wu L, Dong A, Dong L, Wang SQ, Li Y. Elife. 2019 Jan 14;8. pii: 43366. doi: 10.7554/eLife.43366. 10.7554/eLife.43366 PubMed 30638447