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IMS-APEX2
(Plasmid #79058)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 79058 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5595
  • Total vector size (bp) 6390
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    APEX
  • Species
    Soybean
  • Insert Size (bp)
    795
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • V5 (N terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IMS-APEX2 was a gift from Alice Ting (Addgene plasmid # 79058 ; http://n2t.net/addgene:79058 ; RRID:Addgene_79058)
  • For your References section:

    Proteomic mapping of the human mitochondrial intermembrane space in live cells via ratiometric APEX tagging. Hung V, Zou P, Rhee HW, Udeshi ND, Cracan V, Svinkina T, Carr SA, Mootha VK, Ting AY. Mol Cell. 2014 Jul 17;55(2):332-41. doi: 10.1016/j.molcel.2014.06.003. Epub 2014 Jul 4. 10.1016/j.molcel.2014.06.003 PubMed 25002142