pcDNA3 APEX2-NES
(Plasmid #49386)

• Purpose: Expresses APEX2 in the cytosol of mammalian cells
• Depositing Lab: Alice Ting
• Publication: Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5385
• Total vector size (bp): 6198
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Flag - APEX2 - NES
• Species: soybean
• Insert Size (bp): 813
• Mutation: K14D, W41F, E112K, A134P on soybean APX; improved for proteomics and EM applications
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMVf

Resource Information

• Supplemental Documents:
  • APEX2 Recommendations from Ting Lab
• Articles Citing this Plasmid:
  • 58 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry