Addgene - p258 pSP72 hAR-LBD Plasmid Data

Gene/insert name:
AR LBD
Alt name:
androgen receptor
Insert size:
903
Species:
H. sapiens (human)
Entrez Gene:
AR (RP11-383C12.1, AIS, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
Mutation:
AR ligand binding domain
Vector backbone:
pSP72
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Bacterial Expression
Backbone size w/o insert:
2500
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
BglII
Site destroyed during cloning:
Yes
5' sequencing primer:
SP6 List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Jeffrey Green
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Induction of cre recombinase activity using modified androgen receptor ligand binding domains: a sensitive assay for ligand-receptor interactions. Kaczmarczyk et al (Nucleic Acids Res. 2003 Aug 1. 31(15):e86. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12492" in your Materials and Methods section.