p264 pSP72 hAR-LBD(LNCaP)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12495||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2500
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameAR LBD
Alt nameandrogen receptor
SpeciesH. sapiens (human)
Insert Size (bp)903
MutationThr 878 Ala in AR-LBD
Entrez GeneAR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
Plasmid p258 was
digested with BglI and BsgI and the 1.8 kb fragment was
isolated. A second aliquot of p258 was digested with BglI and
DraIII and the 1.3 kb fragment was isolated. Both of these
fragments were ligated to a double-stranded oligonucleotide
resulting in p264.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p264 pSP72 hAR-LBD(LNCaP) was a gift from Jeffrey Green (Addgene plasmid # 12495 ; http://n2t.net/addgene:12495 ; RRID:Addgene_12495)
For your References section:Induction of cre recombinase activity using modified androgen receptor ligand binding domains: a sensitive assay for ligand-receptor interactions. Kaczmarczyk SJ, Green JE. Nucleic Acids Res. 2003 Aug 1. 31(15):e86. 10.1093/nar/gng087 PubMed 12888538