Addgene - EGFP-LC3 Plasmid Data

Plasmid 11546: EGFP-LC3

Gene/insert name:
EGFP-LC3B
Alt name:
NM_022818
Alt name:
Human homolog of yeast ATG8p
Insert size:
380
Species:
H. sapiens (human)
GenBank ID:
NM_022818
Entrez Gene:
MAP1LC3B (ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
Fusion protein or tag:
EGFP
Terminal:
N terminal on insert
Vector backbone:
pEGFP-C3
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4727
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
CMV Forward List of Sequencing Primers
3' sequencing primer:
EBV Reverse
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
Kopito Lab - see reference
Sequence: View sequences (2)
Principal Investigator:
Karla Kirkegaard
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Subversion of cellular autophagosomal machinery by RNA viruses. Jackson et al (PLoS Biol. 2005 May . 3(5):e156. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11546" in your Materials and Methods section.