|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21075||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4500
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Gene/Insert namemicrotubule-associated protein 1 light chain 3 beta
SpeciesR. norvegicus (rat)
Insert Size (bp)738
MutationFirst ATG is not included.
Entrez GeneMap1lc3b (a.k.a. LC3B, Map1lc3, Mpl3, zbs559)
/ Fusion Protein
- mRFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
Compbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharian DA, Tsien RY, A monomeric red fluorescent protein, Proc Natl Acad Sci USA. 2002, 99(12):7877-82
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pmRFP-LC3 was a gift from Tamotsu Yoshimori (Addgene plasmid # 21075 ; http://n2t.net/addgene:21075 ; RRID:Addgene_21075)
For your References section:Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3. Kimura S, Noda T, Yoshimori T. Autophagy. . 3(5):452-60. PubMed 17534139