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pTagGFP2-N SIN1 deltaCRIM
(Plasmid #124921)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 124921 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTagGFP2-N
  • Backbone manufacturer
    Evrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MAPKAP1
  • Alt name
    SIN1
  • Alt name
    mSIN1
  • Species
    H. sapiens (human)
  • Mutation
    Insert ORF was sirently mutated to be resistent to sgMAPKAP1_1.
  • Entrez Gene
    MAPKAP1 (a.k.a. JC310, MIP1, SIN1, SIN1b, SIN1g)
  • Tag / Fusion Protein
    • TagGFP2 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer TagGFP-Rev, GCGCACGCTGAACTTGTGGC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTagGFP2-N SIN1 deltaCRIM was a gift from Jin Chen (Addgene plasmid # 124921 ; http://n2t.net/addgene:124921 ; RRID:Addgene_124921)
  • For your References section:

    Disruption of scaffolding function of mLST8 selectively inhibits mTORC2 assembly and function and suppresses mTORC2-dependent tumor growth in vivo. Yoonha Hwang, Laura C Kim, Wenqiang Song, Deanna N Edwards, Rebecca S. Cook and Jin Chen. Cancer Research, May 2019 10.1158/0008-5472.CAN-18-3658