|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||124919||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
MutationInsert ORF was sirently mutated to be resistent to sgMAPKAP1_1.
Entrez GeneMAPKAP1 (a.k.a. JC310, MIP1, SIN1, SIN1b, SIN1g)
/ Fusion Protein
- TagGFP2 (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer TagGFP-Rev, GCGCACGCTGAACTTGTGGC (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTagGFP2-N SIN1 was a gift from Jin Chen (Addgene plasmid # 124919 ; http://n2t.net/addgene:124919 ; RRID:Addgene_124919)
For your References section:Disruption of scaffolding function of mLST8 selectively inhibits mTORC2 assembly and function and suppresses mTORC2-dependent tumor growth in vivo. Yoonha Hwang, Laura C Kim, Wenqiang Song, Deanna N Edwards, Rebecca S. Cook and Jin Chen. Cancer Research, May 2019 10.1158/0008-5472.CAN-18-3658