Purpose(Empty Backbone) Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BsmBI cloning sites and puromycin selection.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||125518||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 8523
Vector typeMammalian Expression, Lentiviral, CRISPR
- Promoter U6
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer U6 fwd (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
5' cloning site: BsmBI (Esp3I) (destroyed during cloning), 3' cloning site: BsmBI (Esp3I) (destroyed during cloning)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLCKO2 was a gift from Jason Moffat (Addgene plasmid # 125518 ; http://n2t.net/addgene:125518 ; RRID:Addgene_125518)
For your References section:Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies. Mair B, Tomic J, Masud SN, Tonge P, Weiss A, Usaj M, Tong AHY, Kwan JJ, Brown KR, Titus E, Atkins M, Chan KSK, Munsie L, Habsid A, Han H, Kennedy M, Cohen B, Keller G, Moffat J. Cell Rep. 2019 Apr 9;27(2):599-615.e12. doi: 10.1016/j.celrep.2019.02.041. 10.1016/j.celrep.2019.02.041 PubMed 30970261