LNCX TbetaRII DN
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12640||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6600
Vector typeMammalian Expression, Retroviral
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameTGF-beta receptor II
SpeciesH. sapiens (human)
Insert Size (bp)600
MutationDominant negative: truncated cytoplasmically
Entrez GeneTGFBR2 (a.k.a. AAT3, FAA3, LDS1B, LDS2, LDS2B, MFS2, RIIC, TAAD2, TBR-ii, TBRII, TGFR-2, TGFbeta-RII)
/ Fusion Protein
- Flag (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HpaI (destroyed during cloning)
- 3′ cloning site HpaI (destroyed during cloning)
- 5′ sequencing primer LNCX (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LNCX TbetaRII DN was a gift from Rik Derynck (Addgene plasmid # 12640 ; http://n2t.net/addgene:12640 ; RRID:Addgene_12640)
For your References section:Roles of autocrine TGF-beta receptor and Smad signaling in adipocyte differentiation. Choy L, Skillington J, Derynck R. J Cell Biol. 2000 May 1. 149(3):667-82. 10.1083/jcb.149.3.667 PubMed 10791980
Map uploaded by the depositor.