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(Plasmid #126609)


Item Catalog # Description Quantity Price (USD)
Plasmid 126609 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    tRNA ligase 1
  • Alt name
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
  • Entrez Gene
    TRL1 (a.k.a. YJL087C, LIG1, RLG1)
  • Promoter CAG
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Construct contains a non-canonical FLAG tag of DYKDDDDR rather than the typical DYKDDDDK that is functional in the experience of the depositor.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB-bsd-Flag-Trl1 was a gift from Xiaozhong Wang (Addgene plasmid # 126609 ; ; RRID:Addgene_126609)
  • For your References section:

    The cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune noncanonical XBP1 splicing during ER stress. Unlu I, Lu Y, Wang X. J Biol Chem. 2018 Dec 14;293(50):19365-19376. doi: 10.1074/jbc.RA118.004872. Epub 2018 Oct 24. 10.1074/jbc.RA118.004872 PubMed 30355738