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pICSL4723_ WRKY28_1
(Plasmid #126887)

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  • Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
  • Depositing Lab
    Sophien Kamoun
  • Depositing Organization
    Sainsbury Laboratory, Norwich
    Located in United Kingdom of Great Britain and Northern Ireland
  • Publication
    Genome editing in wheat (unpublished) ( How to cite )
  • Sequence Information

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126887 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pICSL4723
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Wheat_live_Cas9
  • gRNA/shRNA sequence
    ACGTCGGCCTCCCGCCGATG, GCAGCTCAAGAAGGACCCTG, GGGCAGACTTCCCTCAGCCT, GGCGCGTGGGCGAGGAGAAC
  • Species
    Synthetic
  • Insert Size (bp)
    8726

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Encodes gene specific sgRNAs driven by the wheat U6 promoter; encodes the plant selectable marker nptII driven by the rice Act1 promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pICSL4723_ WRKY28_1 was a gift from Sophien Kamoun (Addgene plasmid # 126887 ; http://n2t.net/addgene:126887 ; RRID:Addgene_126887)
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